Metabolic dysfunction-associated steatotic liver disease (MASLD) parallels the obesity epidemic in Australia and contributes to rising morbidity and mortality [1, 2]. Improving disease knowledge and health literacy may support lifestyle modification in this population [3, 4]. This study aims to assess disease knowledge and health literacy among Australians with MASLD and explore their association with lifestyle behaviours. This was an online cross-sectional national survey, with participants recruited via social media platforms, liver health-related websites, and tertiary clinics. Adults aged ≥18 years with a self-reported diagnosis of MASLD and no other known cause of steatogenic liver disease and without psychological disorder(s) were eligible for inclusion. Following informed consent, participants completed validated questionnaires assessing disease knowledge, health literacy, physical activity, and sleep quality. Dietary intake was evaluated using the Cancer Council Victoria food frequency questionnaire and diet quality was assessed using the Mediterranean Diet Score (MedDietScore). The survey was administered online via Qualtrics, and personalised links for dietary intake assessment were emailed to eligible participants. Binary and ordinal logistic regression analyses were used to assess the associations between disease knowledge, health literacy levels, and lifestyle behaviours. A total of 93 participants completed the demographics, while 91 and 86 participants completed the disease knowledge and health literacy questionnaires, respectively. The mean age was 63.9 ± 10.9 years, and 91.4% were females. Only 6.6% of participants were aware of the threshold of liver fat required for a MASLD diagnosis (>5%). Most participants (~80%) reported good knowledge of the recommended lifestyle interventions for MASLD management, but 47.7% incorrectly identified excessive alcohol intake as a risk factor for MASLD, and only 47.3% were aware of its cardiovascular disease consequences. More than half (58.2%) exhibited either inadequate or problematic health literacy, particularly in the ability to appraise health information. Disease knowledge was inversely associated with BMI, with each one-unit increase in BMI linked to an 8% lower likelihood of having high disease knowledge (p = 0.041). Disease knowledge and health literacy were not associated with overall diet quality. However, each one-unit increase in legume intake score was associated with a 35% higher likelihood of high disease knowledge (p = 0.039). Health literacy increased with physical activity, with each additional minute of daily activity associated with significantly higher odds of greater health literacy (OR: 1.01; 95% CI: 1.01 – 1.02). Higher health literacy was 61% less likely among participants with sleep disturbances (OR: 0.39; 95% CI: 0.15 – 0.94). This national survey of Australian adults with self‑reported MASLD found moderate disease‑related knowledge and limited health literacy, with both associated with lifestyle behaviours. These findings highlight the need for educational strategies that enhance disease knowledge and ability to appraise health‑related information, supporting adherence to lifestyle modifications in this population.